Spheroids and organoids are in vitro models of organs – much smaller and simpler, but easy to produce and thus for developmental biologists an invaluable tool to study organ development. Unlike cell cultures, which usually consist of a monolayer of cells only, cells in spheroids/organoids form three-dimensional structures, allowing for the investigation of cell migration and differentiation inside 3D cell models. With lattice light sheet microscopy, imaging the development and self-organization